Caspase-5 Recombinant Rabbit Monoclonal Antibody [SD203-2]
cat.: ET1612-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SD203-2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Caspase 5 aa 1-50 / 434. |
| Positive control: | THP-1 cell lysate, HeLa cell lysate, A549 cell lysate, SHG-44 cell lysate, human liver tissue. |
| Subcellular location: | NLRP1 inflammasome complex, cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:5,000 1:50 1:50 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P51878 Human |
| Alternative names: | Caspase5 Apoptosis related cysteine protease CASP-5 CASP5 CASP5_HUMAN Caspase-5 subunit p10 Caspase-5 Caspase 5 Caspase5 ICE(rel)-III ICERELIII ICH 3 ICH 3 protease Protease ICH-3 Protease TY TY TY protease |
Images
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Fig1:
Western blot analysis of Caspase-5 on different lysates with Rabbit anti-Caspase-5 antibody (ET1612-29) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: SHG-44 cell lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-29) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caspase-5 antibody (ET1612-29) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-29) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.