ET1612-33

AP2M1 Recombinant Rabbit Monoclonal Antibody [SD0746]

cat.: ET1612-33

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	SD0746

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	50 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human AP2M1 aa 351-400 / 435.
Positive control:	PC-3M cell lysates, HepG2, SH-SY5Y, MCF-7, human kidney tissue, mouse brain tissue, PANC-1.
Subcellular location:	Cell membrane, coated pit.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: Q96CW1 Human \| P84091 Mouse \| P84092 Rat
Alternative names:	Adapter-related protein complex 2 mu subunit Adaptin mu 1 Adaptin-mu2 Adaptor protein complex AP 2 subunit mu Adaptor protein complex AP-2 subunit mu Adaptor related protein complex 2 mu 1 subunit AP 2 mu 2 chain AP-2 complex subunit mu AP-2 mu chain Ap2m1 AP2M1_HUMAN AP50 CLAPM1 Clathrin adaptor complex AP2 mu subunit Clathrin assembly protein complex 2 medium chain Clathrin associated/assembly/adaptor protein medium 1 Clathrin coat adaptor protein AP50 Clathrin coat assembly protein AP50 Clathrin coat associated protein AP50 Clathrin coat-associated protein AP50 HA2 50 kDa subunit mu2 Plasma membrane adaptor AP-2 50 kDa protein

Images

	Fig1: Western blot analysis of AP2M1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining of AP2M1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of AP2M1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: ICC staining of AP2M1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2M1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1612-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AP2M1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1612-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of AP2M1 was done on PANC-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-33, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.