MEK5 Recombinant Rabbit Monoclonal Antibody [SD208-6]
cat.: ET1612-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SD208-6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MEK5 aa 381-330 / 448. |
| Positive control: | Hela cell lysates, Hela, A431, SW480. |
| Subcellular location: | Spindle, Cytosol, nucleus, cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q13163 Human |
| Alternative names: | Dual specificity mitogen activated protein kinase kinase 5 Dual specificity mitogen-activated protein kinase kinase 5 EC 2.7.12.2 HsT17454 MAP kinase kinase 5 MAP kinase kinase MEK5b MAP2K5 MAPK/ERK kinase 5 MAPKK 5 MAPKK5 MEK 5 mitogen-activated protein kinase kinase 5 MKK5 MP2K5_HUMAN PRKMK5 Protein kinase, mitogen-activated, kinase 5 SAPKK5 SKK5 |
Images
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Fig1: Western blot analysis of MEK5 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of MEK5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of MEK5 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: ICC staining of MEK5 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.