IRF6 Recombinant Rabbit Monoclonal Antibody [SD202-6]
cat.: ET1612-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD202-6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein Human IRF6 aa 364-467 / 467. |
| Positive control: | K-562 cell lysate, mouse kidney tissue, human kidney tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: O14896 Human | P97431 Mouse Entrez Gene: 364081 Rat |
| Alternative names: | Interferon regulatory factor 6 IRF 6 IRF-6 IRF6 IRF6_HUMAN LPS OFC 6 OFC6 OTTHUMP00000034677 OTTHUMP00000034678 PIT PPS PPS1 VWS VWS1 |
Images
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Fig1:
Western blot analysis of IRF6 on different lysates with Rabbit anti-IRF6 antibody (ET1612-36) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate (negative) Lane 2: HaCaT cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 60 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-36) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.