Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
Clonality: | Monoclonal |
Clone number: | SD202-6 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 60 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein Human IRF6 aa 364-467 / 467. |
Positive control: | K562, MCF-7, 293, SW480, human kidney tissue, mouse kidney tissue. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: O14896 Human | P97431 Mouse Entrez Gene: 364081 Rat |
Alternative names: | Interferon regulatory factor 6 IRF 6 IRF-6 IRF6 IRF6_HUMAN LPS OFC 6 OFC6 OTTHUMP00000034677 OTTHUMP00000034678 PIT PPS PPS1 VWS VWS1 |
Fig1: Western blot analysis of IRF6 on K562 cells lysates using anti-IRF6 antibody at 1/1,000 dilution. | |
Fig2: ICC staining IRF6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining IRF6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining IRF6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF6 antibody. Counter stained with hematoxylin. |