Cyclin D3 Recombinant Rabbit Monoclonal Antibody [SD20-43]
cat.: ET1612-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD20-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cyclin D3 aa 162-292 / 292. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, A20 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P30281 Human | P30282 Mouse | P48961 Rat |
| Alternative names: | CCND 3 Ccnd3 CCND3_HUMAN CyclinD3 D3 type cyclin G1 S specific cyclin D3 G1/S specific cyclin D3 G1/S-specific cyclin-D3 |
Images
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Fig1:
Western blot analysis of Cyclin D3 on different lysates with Rabbit anti-Cyclin D3 antibody (ET1612-4) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A20 cell lysate Lane 5: C2C12 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.