Anti-14-3-3 sigma antibody [SD2070]
cat.: ET1612-41
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SD2070
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human 14-3-3 sigmaaa 50-99 / 248.
Positive control: Human tonsil tissue, human breast carcinoma tissue.
Subcellular location: Cytoplasm, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:50-1:200
Uniprot #: SwissProt: P31947 Human
Alternative names: 14 3 3 protein sigma 14-3-3 protein sigma 1433S_HUMAN Epithelial cell marker protein 1 Er HME 1 HME1 MGC143283 Mkrn3 Mme1 OTTHUMP00000004242 RP23 137L22.11 SFN SFN protein Stratifin YWHAS
Images
ET1612-41_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-14-3-3 sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-41_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-41, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.