Phospho-MEK1 (T292) Recombinant Rabbit Monoclonal Antibody [SD2088]
cat.: ET1612-42
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SD2088
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr292 of Human MEK1 aa 271-320 / 393.
Positive control: HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, PC-12 cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, mouse brain tissue.
Subcellular location: Cytoplasm, Nucleus, Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:200
Uniprot #: SwissProt: Q02750 Human | P31938 Mouse | Q01986 Rat
Alternative names: Dual specificity mitogen activated protein kinase kinase 1 Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAP kinase kinase 1 MAP kinase/Erk kinase 1 MAP2K1 MAPK/ERK kinase 1 MAPKK 1 MAPKK1 MEK 1 Mek1 MEKK1 Mitogen activated protein kinase kinase 1 MKK 1 MKK1 MP2K1_HUMAN PRKMK1 Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) Protein kinase mitogen activated, kinase 1
Images
ET1612-42_1.jpg Fig1: Western blot analysis of Phospho-MEK1 (T292) on different lysates with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: PC-12 cell lysate
Lane 4: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate
Lane 5: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 6: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 7: NIH/3T3 cell lysate
Lane 8: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate
Lane 9: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour

Lysates/proteins at 20 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-42_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.