Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IP, FC |
Clonality: | Monoclonal |
Clone number: | SD202-09 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 38 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C-terminal Human CEBP alpha aa 309-358 / 358. |
Positive control: | Hela. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB FC IP |
1:1,000 1:50-1:100 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P49715 Human | P53566 Mouse | P05554 Rat |
Alternative names: | Apoptotic cysteine protease Apoptotic protease Mch 5 C/EBP alpha C/ebpalpha CAP4 Caspase 8 precursor CBF-A CCAAT Enhancer Binding Protein alpha CCAAT/enhancer binding protein (C/EBP), alpha CCAAT/enhancer-binding protein alpha CEBP CEBP A CEBP alpha Cebpa CEBPA_HUMAN FADD homologous ICE/CED 3 like protease FADD like ICE FLICE ICE like apoptotic protease 5 ICE8 MACH MCH5 MORT1 associated CED 3 homolog |
Fig1: Flow cytometric analysis of CEBP alpha was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-46, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |