CEBP alpha Recombinant Rabbit Monoclonal Antibody [SD202-09]
cat.: ET1612-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SD202-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human CEBP alpha aa 309-358 / 358. |
| Positive control: | THP-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB IP |
1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P49715 Human | P53566 Mouse | P05554 Rat |
| Alternative names: | Apoptotic cysteine protease Apoptotic protease Mch 5 C/EBP alpha C/ebpalpha CAP4 Caspase 8 precursor CBF-A CCAAT Enhancer Binding Protein alpha CCAAT/enhancer binding protein (C/EBP), alpha CCAAT/enhancer-binding protein alpha CEBP CEBP A CEBP alpha Cebpa CEBPA_HUMAN FADD homologous ICE/CED 3 like protease FADD like ICE FLICE ICE like apoptotic protease 5 ICE8 MACH MCH5 MORT1 associated CED 3 homolog |
Images
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Fig1:
Western blot analysis of CEBP alpha on different lysates with Rabbit anti-CEBP alpha antibody (ET1612-46) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: Mouse liver tissue lysate (30 µg/Lane) Lane 3: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 38 kDa Observed band size: 30/42 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.