Angiotensinogen Recombinant Rabbit Monoclonal Antibody [SD201-02]
cat.: ET1612-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | SD201-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein Human Angiotensinogen aa 361-476. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, Huh7 (Human liver cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, LO2, 293, Hela, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P01019 Human |
| Alternative names: | Aangiotensinogen (serpin peptidase inhibitor clade A member 8) AGT AI265500 Alpha 1 antiproteinase antitrypsin Ang Ang I Ang II Ang III AngII Angiotensin I Angiotensin II Angiotensin III Angiotensin-3 Angiotensinogen (PAT) Angiotensinogen ANGT_HUMAN ANHU ANRT AT-2 AT-II Des-Asp[1]-angiotensin II FLJ92595 FLJ97926 MGC105326 PAT Pre angiotensinogen Serine (or cysteine) proteinase inhibitor Serpin A8 Serpin peptidase inhibitor clade A member 8 SERPINA8 |
Images
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Fig1:
Western blot analysis of Angiotensinogen on different lysates with Rabbit anti-Angiotensinogen antibody (ET1612-48) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: Huh7 (Human liver cancer cell) cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1612-48, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
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Fig2: ICC staining Angiotensinogen in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Angiotensinogen in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining Angiotensinogen in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.