RUNX1+RUNX2+RUNX3 Recombinant Rabbit Monoclonal Antibody [SD0803]
cat.: ET1612-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SD0803 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 49 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RUNX1 aa 404-453 / 453. |
| Positive control: | Jurkat cell lysates, SHG-44, human tonsil tissue, Jurkat. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q01196 Human | Q13761 Human | Q13950 Human | Q03347 Mouse | Q08775 Mouse | Q64131 Mouse | Q63046 Rat | Q9Z2J9 Rat |
| Alternative names: | Runt-related transcription factor 1 Acute myeloid leukemia 1 protein Core-binding factor subunit alpha-2 CBF-alpha-2 Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit PEA2-alpha B PEBP2-alpha B SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit RUNX1 AML1 CBFA2 Runt-related transcription factor 2 Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 CBF-alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 OSF-2 Polyomavirus enhancer-binding protein 2 alpha A subunit PEA2-alpha A PEBP2-alpha A SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit RUNX2 AML3 CBFA1 OSF2 PEBP2A Runt-related transcription factor 3 Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 CBF-alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit PEA2-alpha C PEBP2-alpha C SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core-...... |
Images
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Fig1: Western blot analysis of RUNX1+RUNX2+RUNX3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of RUNX1+RUNX2+RUNX3 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of RUNX1+RUNX2+RUNX3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.