RUNX1+RUNX2+RUNX3 Recombinant Rabbit Monoclonal Antibody [SD0803]
cat.: ET1612-49
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SD0803
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 49 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RUNX1 aa 404-453 / 453.
Positive control: Saos-2 cell lysate, NIH/3T3 cell lysate, SHG-44 cell lysate, Mouse thymus tissue lysate, SHG-44, mouse testis tissue, human tonsil tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:1:1,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q01196 Human | Q13761 Human | Q13950 Human | Q03347 Mouse | Q08775 Mouse | Q64131 Mouse | Q63046 Rat | Q9Z2J9 Rat
Alternative names: Runt-related transcription factor 1 Acute myeloid leukemia 1 protein Core-binding factor subunit alpha-2 CBF-alpha-2 Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit PEA2-alpha B PEBP2-alpha B SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit RUNX1 AML1 CBFA2 Runt-related transcription factor 2 Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 CBF-alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 OSF-2 Polyomavirus enhancer-binding protein 2 alpha A subunit PEA2-alpha A PEBP2-alpha A SL3-3 enhancer factor 1 alpha A subunit SL3/AKV core-binding factor alpha A subunit RUNX2 AML3 CBFA1 OSF2 PEBP2A Runt-related transcription factor 3 Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 CBF-alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit PEA2-alpha C PEBP2-alpha C SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core-......
Images
ET1612-49_1.jpg Fig1: Western blot analysis of RUNX1+RUNX2+RUNX3 on different lysates with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (ET1612-49) at 1/1,000 dilution.

Lane 1: Saos-2 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: SHG-44 cell lysate
Lane 4: Mouse thymus tissue lysate

Lysates/proteins at 10 µg/Lane1-3 and 20ug/Lane4.

Predicted band size: 49 kDa
Observed band size: 50 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-49_2.jpg Fig2: Immunocytochemistry analysis of SHG-44 cells labeling RUNX1+RUNX2+RUNX3 with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (ET1612-49) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (ET1612-49) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1612-49_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.
ET1612-49_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-49_5.jpg Fig5: Flow cytometric analysis of SHG-44 cells labeling RUNX1+RUNX2+RUNX3.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1612-49, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.