Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]
cat.: ET1612-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SD0814 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Stromal aa 561-685 / 685. |
| Positive control: | K-562, HepG2, Human thyroid tissue, mouse skeletal muscle tissue, mouse testis tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Cytoplasm, Sarcoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13586 Human | P70302 Mouse | P84903 Rat |
| Alternative names: | D11S4896E GOK OTTHUMP00000164512 OTTHUMP00000229140 OTTHUMP00000230742 SIM STIM 1 STIM1 Stim1 stromal interaction molecule 1 STIM1_HUMAN STIM1L Stromal interaction molecule 1 |
Images
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Fig1:
Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.