Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]
cat.: ET1612-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SD0814
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 100 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Stromal aa 561-685 / 685.
Positive control: K-562, HepG2, Human thyroid tissue, mouse skeletal muscle tissue, mouse testis tissue.
Subcellular location: Cell membrane, Endoplasmic reticulum membrane, Cytoplasm, Sarcoplasmic reticulum.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:50-1:200
Uniprot #: SwissProt: Q13586 Human | P70302 Mouse | P84903 Rat
Alternative names: D11S4896E GOK OTTHUMP00000164512 OTTHUMP00000229140 OTTHUMP00000230742 SIM STIM 1 STIM1 Stim1 stromal interaction molecule 1 STIM1_HUMAN STIM1L Stromal interaction molecule 1
Images
ET1612-53_1.jpg Fig1: Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: HepG2
ET1612-53_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-53_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-53_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.