Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814]
cat.: ET1612-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SD0814 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Stromal aa 561-685 / 685. |
| Positive control: | SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Human lung tissue lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, human thyroid tissue, mouse skeletal muscle tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Cytoplasm, Sarcoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:500 |
| Uniprot #: | SwissProt: Q13586 Human | P70302 Mouse | P84903 Rat |
| Alternative names: | D11S4896E GOK OTTHUMP00000164512 OTTHUMP00000229140 OTTHUMP00000230742 SIM STIM 1 STIM1 Stim1 stromal interaction molecule 1 STIM1_HUMAN STIM1L Stromal interaction molecule 1 |
Images
|
Fig1:
Western blot analysis of Stromal interaction molecule 1 on different lysates with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: Human lung tissue lysate (40 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Stromal interaction molecule 1 antibody (ET1612-53) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-53) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.