Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IP |
Clonality: | Monoclonal |
Clone number: | SD08-71 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 75 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Hsp75 aa 560-704 / 704. |
Positive control: | Hela cell lysate, 293T cell lysate. |
Subcellular location: | Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix. |
Recommended Dilutions:
WB |
1:1,000-1:2,000 |
Uniprot #: | SwissProt: Q12931 Human |
Alternative names: | Heat Shock Protein 75 Heat shock protein 75 kDa Heat shock protein 75 kDa mitochondrial HSP 75 HSP90L mitochondrial TNF receptor associated protein 1 TNFR Associated Protein TNFR-associated protein 1 TRAP-1 Trap1 TRAP1_HUMAN Tumor Necrosis Factor Receptor Associated Protein 1 Tumor necrosis factor type 1 receptor associated protein Tumor necrosis factor type 1 receptor-associated protein |
Fig1:
Western blot analysis of Hsp75 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate |