Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71]
cat.: ET1612-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP
Clonality: Monoclonal
Clone number: SD08-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 75 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Hsp75 aa 560-704 / 704.
Positive control: Hela cell lysate, 293T cell lysate.
Subcellular location: Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix.
Recommended Dilutions:
  WB

1:1,000-1:2,000
Uniprot #: SwissProt: Q12931 Human
Alternative names: Heat Shock Protein 75 Heat shock protein 75 kDa Heat shock protein 75 kDa mitochondrial HSP 75 HSP90L mitochondrial TNF receptor associated protein 1 TNFR Associated Protein TNFR-associated protein 1 TRAP-1 Trap1 TRAP1_HUMAN Tumor Necrosis Factor Receptor Associated Protein 1 Tumor necrosis factor type 1 receptor associated protein Tumor necrosis factor type 1 receptor-associated protein
Images
ET1612-54_1.jpg Fig1: Western blot analysis of Hsp75 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.