Anti-KAP1 antibody [SD081-05]
cat.: ET1612-55
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: SD081-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 110 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human KAP1 aa 310-350.
Positive control: HepG2 cell lysate, Hela cell lysate, A549, human lung carcinoma tissue, human liver carcinoma tissue, human breast carcinoma tissue, human kidney tissue, human spleen tissue, mouse brain tissue, Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:2,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q13263 Human | Q62318 Mouse | O08629 Rat
Alternative names: E3 SUMO protein ligase TRIM28 antibody E3 SUMO-protein ligase TRIM28 antibody FLJ29029 antibody KAP 1 antibody KAP-1 antibody KRAB associated protein 1 antibody KRAB interacting protein 1 antibody KRAB-associated protein 1 antibody KRAB-interacting protein 1 antibody KRIP 1 antibody KRIP-1 antibody KRIP1 antibody Nuclear corepressor KAP 1 antibody Nuclear corepressor KAP-1 antibody RING finger protein 96 antibody RNF96 antibody TF1B antibody TIF1 beta antibody TIF1-beta antibody TIF1B antibody TIF1B_HUMAN antibody Transcription intermediary factor 1 beta antibody Transcription intermediary factor 1-beta antibody Trim28 antibody Tripartite motif containing 28 antibody tripartite motif containing protein 28 antibody Tripartite motif-containing protein 28 antibody
Images
ET1612-55_1.jpg Fig1: Western blot analysis of KAP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
ET1612-55_2.jpg Fig2: ICC staining of KAP1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-55_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KAP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-55_9.jpg Fig9: Flow cytometric analysis of KAP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-55, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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