KAP1 Recombinant Rabbit Monoclonal Antibody [SD081-05]
cat.: ET1612-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD081-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KAP1 aa 301-350 / 835. |
| Positive control: | F9 cell lysate, C6 cell lysate, Human lung tissue lysate, Mouse colon tissue lysate, human kidney tissue, mouse brain tissue, rat kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:2,000-1:5,000 1:50-1:200 1:3,000 |
| Uniprot #: | SwissProt: Q13263 Human | Q62318 Mouse | O08629 Rat |
| Alternative names: | E3 SUMO protein ligase TRIM28 E3 SUMO-protein ligase TRIM28 FLJ29029 KAP 1 KAP-1 KRAB associated protein 1 KRAB interacting protein 1 KRAB-associated protein 1 KRAB-interacting protein 1 KRIP 1 KRIP-1 KRIP1 Nuclear corepressor KAP 1 Nuclear corepressor KAP-1 RING finger protein 96 RNF96 TF1B TIF1 beta TIF1-beta TIF1B TIF1B_HUMAN Transcription intermediary factor 1 beta Transcription intermediary factor 1-beta Trim28 Tripartite motif containing 28 tripartite motif containing protein 28 Tripartite motif-containing protein 28 |
Images
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Fig1:
Western blot analysis of KAP1 on different lysates with Rabbit anti-KAP1 antibody (ET1612-55) at 1/2,000 dilution. Lane 1: F9 cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Predicted band size: 89 kDa Observed band size: 110 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-55) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.