Anti-PAX6 antibody [SD081-03]
cat.: ET1612-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: SD081-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PAX6 aa 373-422 / 422.
Positive control: K562 cell lysate, Hela cell lysate, rat eyeball tissue, mouse eyeball tissue, human pancreas tissue, SH-SY5Y.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:500-1:2,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P26367 Human | P63015 Mouse | P63016 Rat
Alternative names: AN 2 AN AN2 Aniridia type II protein D11S812E FVH1 KIAA0552 Leucine zipper putative tumor suppressor 3 LZTS3 MGC17209 MGDA Oculorhombin Paired box 6 Paired box gene 6 (aniridia keratitis) Paired Box Gene 6 Paired box homeotic gene 6 Paired box protein Pax-6 Paired box protein Pax6 PAX 6 PAX6 PAX6_HUMAN ProSAP-interacting protein 1 PROSAPIP1 Sey WAGR
Images
ET1612-58_1.jpg Fig1: Western blot analysis of PAX6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Hela cell lysate
ET1612-58_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-PAX6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-58_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue using anti-PAX6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PAX6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-58_5.jpg Fig5: Flow cytometric analysis of PAX6 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-58, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.