PAX9 Recombinant Rabbit Monoclonal Antibody [SD082-02]
cat.: ET1612-59
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD082-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PAX9 aa 6-120 / 341. |
| Positive control: | Mouse skeletal muscle tissue lysate, Mouse stomach tissue lysate, Rat skeletal muscle tissue lysate, Rat heart tissue lysate, HepG2 cell lysate, HeLa cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P55771 Human | P47242 Mouse | Q2L4T2 Rat |
| Alternative names: | Paired box 9 Paired box gene 9 Paired box homeotic gene 9 Paired box protein 9 Paired box protein Pax 9 Paired box protein Pax-9 Paired box protein Pax9 Paired domain gene 9 PAX 9 PAX9 PAX9_HUMAN STHAG3 |
Images
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Fig1:
Western blot analysis of PAX9 on different lysates with Rabbit anti-PAX9 antibody (ET1612-59) at 1/5,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Mouse stomach tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 36 kDa Observed band size: 34 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-59) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PAX9 on different lysates using anti-PAX9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.