Anti-MEK2 antibody [SD207-08]
cat.: ET1612-6
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, ICC/IF, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SD207-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A purified. |
| Molecular weight: | 44 kDa |
| Isotype: | IgG |
| Immunogen: | recombinant protein |
| Positive control: | NIH/3T3, MCF-7, Hela, 293T, human liver cancer tissue, human spleen tissue, human breast carcinoma tissue, mouse lung tissue. |
| Subcellular location: | Cytoplasm, Membrane. |
| Recommended Dilutions:
WB ICC/IF IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | P36507(Human) Q63932(Mouse) |
| Alternative names: | Cardiofaciocutaneous syndrome antibody CFC syndrome antibody CFC4 antibody Dual specificity mitogen activated protein kinase kinase 2 antibody Dual specificity mitogen-activated protein kinase kinase 2 antibody ERK activator kinase 2 antibody FLJ26075 antibody MAP kinase kinase 2 antibody map2k2 antibody MAPK / ERK kinase 2 antibody MAPK/ERK kinase 2 antibody MAPKK 2 antibody MAPKK2 antibody MEK 2 antibody MEK2 antibody Microtubule associated protein kinase kinase 2 antibody Mitogen activated protein kinase kinase 2 antibody Mitogen activated protein kinase kinase 2 p45 antibody MKK 2 antibody MKK2 antibody MP2K2_HUMAN antibody OTTHUMP00000165826 antibody OTTHUMP00000165827 antibody PRKMK 2 antibody PRKMK2 antibody |
Images
|
Fig1:
Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: 293T |
|
Fig2: ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining MEK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin. |
|
Fig9: Flow cytometric analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.