Anti-MEK2 antibody [SD207-08]
cat.: ET1612-6
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SD207-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 44 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: NIH/3T3, MCF-7, Hela, 293T, human liver cancer tissue, human spleen tissue, human breast carcinoma tissue, mouse lung tissue.
Subcellular location: Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:5,000
1:100-1:500
1:100-1:500
1:50-1:100
Uniprot #: P36507(Human) Q63932(Mouse)
Alternative names: Cardiofaciocutaneous syndrome antibody CFC syndrome antibody CFC4 antibody Dual specificity mitogen activated protein kinase kinase 2 antibody Dual specificity mitogen-activated protein kinase kinase 2 antibody ERK activator kinase 2 antibody FLJ26075 antibody MAP kinase kinase 2 antibody map2k2 antibody MAPK / ERK kinase 2 antibody MAPK/ERK kinase 2 antibody MAPKK 2 antibody MAPKK2 antibody MEK 2 antibody MEK2 antibody Microtubule associated protein kinase kinase 2 antibody Mitogen activated protein kinase kinase 2 antibody Mitogen activated protein kinase kinase 2 p45 antibody MKK 2 antibody MKK2 antibody MP2K2_HUMAN antibody OTTHUMP00000165826 antibody OTTHUMP00000165827 antibody PRKMK 2 antibody PRKMK2 antibody
Images
ET1612-6_1.jpg Fig1: Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: 293T
ET1612-6_2.jpg Fig2: ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-6_3.jpg Fig3: ICC staining MEK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-6_4.jpg Fig4: ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-6_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
ET1612-6_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
ET1612-6_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
ET1612-6_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
ET1612-6_9.jpg Fig9: Flow cytometric analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.