MEK2 Recombinant Rabbit Monoclonal Antibody [SD207-08]
cat.: ET1612-6
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SD207-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MEK2 aa 1-50/400.
Positive control: Jurkat cell lysate, Hela cell lysate, 293T cell lysate, Hela, MCF-7, NIH/3T3, human spleen tissue, human breast carcinoma tissue, mouse lung tissue, human liver carcinoma tissue.
Subcellular location: Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000-1:5,000
1:100-1:500
1:100-1:500
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P36507 Human | Q63932 Mouse
Alternative names: Cardiofaciocutaneous syndrome CFC syndrome CFC4 Dual specificity mitogen activated protein kinase kinase 2 Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 FLJ26075 MAP kinase kinase 2 map2k2 MAPK / ERK kinase 2 MAPK/ERK kinase 2 MAPKK 2 MAPKK2 MEK 2 MEK2 Microtubule associated protein kinase kinase 2 Mitogen activated protein kinase kinase 2 Mitogen activated protein kinase kinase 2 p45 MKK 2 MKK2 MP2K2_HUMAN OTTHUMP00000165826 OTTHUMP00000165827 PRKMK 2 PRKMK2
Images
ET1612-6_1.jpg Fig1: Western blot analysis of MEK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate

Predicted band size: 44 kDa
Observed band size: 44 kDa
ET1612-6_2.jpg Fig2: ICC staining of MEK2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-6_3.jpg Fig3: ICC staining of MEK2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-6_4.jpg Fig4: ICC staining of MEK2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-6_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-6_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-6_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-6_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-MEK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-6_9.jpg Fig9: Flow cytometric analysis of MEK2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-6, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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