Anti-PAX8 antibody [SD0832]
cat.: ET1612-61
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SD0832
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 48KD
Isotype: IgG
Immunogen: recombinant protein
Positive control: SKOV-3 cell lysates, SKOV-3, human thyroid tissue, human kidney tissue, mouse kidney tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:2000
1:50-1:200
1:50-1:200
Uniprot #: Q06710(Human) Q00288(Mouse) P51974(Rat)
Alternative names: OTTHUMP00000158659 antibody OTTHUMP00000158660 antibody OTTHUMP00000203723 antibody OTTHUMP00000203724 antibody Paired box 8 antibody Paired box gene 8 antibody paired box homeotic gene 8 antibody Paired box protein Pax 8 antibody Paired box protein Pax-8 antibody Paired domain gene 8 antibody PAX 8 antibody PAX8 antibody PAX8_HUMAN antibody
Images
ET1612-61_1.jpg Fig1: Western blot analysis of PAX8 on SKOV-3 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1612-61_2.jpg Fig2: ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-61_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
ET1612-61_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
ET1612-61_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.