PAX8 Recombinant Rabbit Monoclonal Antibody [SD0832]
cat.: ET1612-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD0832 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48KD |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PAX8 aa 260-450 / 450. |
| Positive control: | SKOV-3 cell lysates, SKOV-3, human thyroid tissue, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q06710 Human | Q00288 Mouse | P51974 Rat |
| Alternative names: | OTTHUMP00000158659 OTTHUMP00000158660 OTTHUMP00000203723 OTTHUMP00000203724 Paired box 8 Paired box gene 8 paired box homeotic gene 8 Paired box protein Pax 8 Paired box protein Pax-8 Paired domain gene 8 PAX 8 PAX8 PAX8_HUMAN |
Images
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Fig1: Western blot analysis of PAX8 on SKOV-3 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.