Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IF-Cell, IF-Tissue |
Clonality: | Monoclonal |
Clone number: | SD08-25 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 92 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human FGFR1 aa 766-822 / 822. |
Positive control: | U-87 MG cell lysate, Hela, 293T. |
Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000 1:50-1:100 1:50-1:100 |
Uniprot #: | SwissProt: P11362 Human |
Alternative names: | Basic fibroblast growth factor receptor 1 bFGF-R-1 BFGFR CD331 CEK FGFBR FGFR 1 FGFR-1 FGFR1 FGFR1/PLAG1 fusion FGFR1_HUMAN fibroblast growth factor receptor 1 FLG FLT-2 FLT2 Fms-like gene Fms-like tyrosine kinase 2 fms-related tyrosine kinase 2 HBGFR heparin-binding growth factor receptor HH2 HRTFDS hydroxyaryl-protein kinase KAL2 N-SAM OGD Proto-oncogene c-Fgr |
Fig1:
Western blot analysis of FGFR1 on different lysates with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 100/140 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of FGFR1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: ICC staining of FGFR1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |