FGFR1 Recombinant Rabbit Monoclonal Antibody [SD08-25]
cat.: ET1612-62
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: SD08-25
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human FGFR1 aa 766-822 / 822.
Positive control: U-87 MG cell lysate, Hela, 293T.
Subcellular location: Cell membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue

1:1,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P11362 Human
Alternative names: Basic fibroblast growth factor receptor 1 bFGF-R-1 BFGFR CD331 CEK FGFBR FGFR 1 FGFR-1 FGFR1 FGFR1/PLAG1 fusion FGFR1_HUMAN fibroblast growth factor receptor 1 FLG FLT-2 FLT2 Fms-like gene Fms-like tyrosine kinase 2 fms-related tyrosine kinase 2 HBGFR heparin-binding growth factor receptor HH2 HRTFDS hydroxyaryl-protein kinase KAL2 N-SAM OGD Proto-oncogene c-Fgr
Images
ET1612-62_1.jpg Fig1: Western blot analysis of FGFR1 on different lysates with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/1,000 dilution.

Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 100/140 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-62_2.jpg Fig2: ICC staining of FGFR1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-62_3.jpg Fig3: ICC staining of FGFR1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-62, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.