FGFR1 Recombinant Rabbit Monoclonal Antibody [SD08-25]
cat.: ET1612-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SD08-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FGFR1 aa 766-822 / 822. |
| Positive control: | U-87 MG cell lysate, U-87 MG. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P11362 Human |
| Alternative names: | Basic fibroblast growth factor receptor 1 bFGF-R-1 BFGFR CD331 CEK FGFBR FGFR 1 FGFR-1 FGFR1 FGFR1/PLAG1 fusion FGFR1_HUMAN fibroblast growth factor receptor 1 FLG FLT-2 FLT2 Fms-like gene Fms-like tyrosine kinase 2 fms-related tyrosine kinase 2 HBGFR heparin-binding growth factor receptor HH2 HRTFDS hydroxyaryl-protein kinase KAL2 N-SAM OGD Proto-oncogene c-Fgr |
Images
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Fig1:
Western blot analysis of FGFR1 on different lysates with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 100/140 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of FGFR1 on different lysates with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-FGFR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 92 kDa Observed band size: 150 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of U-87 MG (Positive) and HT-29 (negative) cells labeling FGFR1 with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FGFR1 antibody (ET1612-62) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.