HDAC7 Recombinant Rabbit Monoclonal Antibody [SD082-4]
cat.: ET1612-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SD082-4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HDAC7 aa 1-218 / 952. |
| Positive control: | A549, Human brain, HeLa, K562. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q8WUI4 Human | Q8C2B3 Mouse | Q99P96 Rat |
| Alternative names: | DKFZP586J0917 FLJ99588 HD 7a HD7 HD7a HDAC 7 HDAC 7A Hdac7 HDAC7_HUMAN HDAC7A Histone deacetylase 7 Histone deacetylase 7A OTTHUMP00000202813 OTTHUMP00000202814 |
Images
|
Fig1:
Immunocytochemistry analysis of HeLa cells labeling HDAC7 with Rabbit anti-HDAC7 antibody (ET1612-67) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HDAC7 antibody (ET1612-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Western blot analysis of HDAC7 on Hela lysates with Rabbit anti-HDAC7 antibody (ET1612-67) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1612-67, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 103 kDa Observed band size: 103 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.