JNK3 Recombinant Rabbit Monoclonal Antibody [SD082-09]
cat.: ET1612-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | SD082-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human JNK3 aa 401-450 / 464. |
| Positive control: | 293T, NIH/3T3, Hela, SH-SY-5Y. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB FC IF-Cell IF-Tissue |
1:500 1:50-1:100 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: P53779 Human | Q61831 Mouse | P49187 Rat |
| Alternative names: | c Jun kinase 3 c-Jun N-terminal kinase 3 cJun N terminal kinase 3 FLJ12099 FLJ33785 JNK3 alpha protein kinase JNK3 JNK3A MAP kinase 10 MAP kinase MAP kinase p49 3F12 MAPK 10 Mapk10 MGC50974 mitogen activated protein kinase 10 Mitogen-activated protein kinase 10 MK10_HUMAN p493F12 p54bSAPK PRKM10 protein kinase mitogen activated 10 SAPK1b Stress activated protein kinase 1b stress activated protein kinase beta Stress activated protein kinase JNK3 Stress-activated protein kinase JNK3 |
Images
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Fig1: Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution. |
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Fig2: ICC staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining JNK3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of Jurkat cells with JNK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.