JNK3 Recombinant Rabbit Monoclonal Antibody [SD082-09]
cat.: ET1612-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD082-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human JNK3 aa 401-450 / 464. |
| Positive control: | MCF7 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:5,000 1:100-1:500 1:200 |
| Uniprot #: | SwissProt: P53779 Human | Q61831 Mouse | P49187 Rat |
| Alternative names: | c Jun kinase 3 c-Jun N-terminal kinase 3 cJun N terminal kinase 3 FLJ12099 FLJ33785 JNK3 alpha protein kinase JNK3 JNK3A MAP kinase 10 MAP kinase MAP kinase p49 3F12 MAPK 10 Mapk10 MGC50974 mitogen activated protein kinase 10 Mitogen-activated protein kinase 10 MK10_HUMAN p493F12 p54bSAPK PRKM10 protein kinase mitogen activated 10 SAPK1b Stress activated protein kinase 1b stress activated protein kinase beta Stress activated protein kinase JNK3 Stress-activated protein kinase JNK3 |
Images
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Fig1:
Western blot analysis of JNK3 on different lysates with Rabbit anti-JNK3 antibody (ET1612-68) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-68) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK3 antibody (ET1612-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK3 antibody (ET1612-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK3 antibody (ET1612-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.