Anti-JNK3 antibody [SD082-09]
cat.: ET1612-68
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, FC
Clonality: Monoclonal
Clone number: SD082-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human JNK3 aa 401-450 / 464.
Positive control: 293T, NIH/3T3, Hela, SH-SY-5Y.
Subcellular location: Cytoplasm, Membrane, Nucleus, Mitochondrion.
Recommended Dilutions:
  WB
  FC
  ICC/IF

1:500
1:50-1:100
1:100-1:500
Uniprot #: SwissProt: P53779 Human | Q61831 Mouse | P49187 Rat
Alternative names: c Jun kinase 3 c-Jun N-terminal kinase 3 cJun N terminal kinase 3 FLJ12099 FLJ33785 JNK3 alpha protein kinase JNK3 JNK3A MAP kinase 10 MAP kinase MAP kinase p49 3F12 MAPK 10 Mapk10 MGC50974 mitogen activated protein kinase 10 Mitogen-activated protein kinase 10 MK10_HUMAN p493F12 p54bSAPK PRKM10 protein kinase mitogen activated 10 SAPK1b Stress activated protein kinase 1b stress activated protein kinase beta Stress activated protein kinase JNK3 Stress-activated protein kinase JNK3
Images
ET1612-68_1.jpg Fig1: Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution.
ET1612-68_2.jpg Fig2: ICC staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-68_3.jpg Fig3: ICC staining JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-68_4.jpg Fig4: ICC staining JNK3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1612-68_5.jpg Fig5: Flow cytometric analysis of Jurkat cells with JNK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.