Caspase-8 Recombinant Rabbit Monoclonal Antibody [SD08-06]
cat.: ET1612-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SD08-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Caspase-8 aa 200-249 / 479.
Positive control: Jurkat cell lysate, HeLa cell lysate, THP-1 cell lysate, HepG2 cell lysate, 293T cell lysate, human tonsil tissue, human spleen tissue, human lung carcinoma tissue, human kidney tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Tissue
  IHC-P
1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q14790 Human
Alternative names: ALPS2B Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein Apoptotic cysteine protease Apoptotic protease Mch-5 Apoptotic protease Mch5 CAP4 CASP-8 CASP8 CASP8_HUMAN Caspase8 Caspase 8 Caspase 8 apoptosis related cysteine peptidase Caspase-8 subunit p10 CED 3 FADD Like ICE FADD-homologous ICE/CED-3-like protease FADD-like ICE FLICE FLJ17672 ICE-like apoptotic protease 5 MACH alpha 1/2/3 protein MACH MACH beta 1/2/3/4 protein MCH5 MGC78473 MORT1 associated ced 3 homolog MORT1-associated CED-3 homolog OTTHUMP00000163717 OTTHUMP00000163720 OTTHUMP00000163724 OTTHUMP00000163725 OTTHUMP00000165062 OTTHUMP00000165063 OTTHUMP00000165064 OTTHUMP00000206552 OTTHUMP00000206582
Images
ET1612-70_1.jpg Fig1: Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (ET1612-70) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate
Lane 3: THP-1 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: 293T cell lysate
Lane 6: SH-SY5Y cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 55/54 kDa

Exposure time: 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-70_2.jpg Fig2: Western blot analysis of Caspase-8 on different lysates with Rabbit anti-Caspase-8 antibody (ET1612-70) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Caspase-8 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 54,55 kDa

Exposure time: 180 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-70_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.