Anti-Caspase-8 antibody [SD08-06]
cat.: ET1612-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SD08-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Caspase-8 aa 200-250.
Positive control: Jurkat cell lysates, Hela, A549, human tonsil tissue, human spleen tissue, human lung carcinoma tissue, human kidney tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q14790 Human
Alternative names: ALPS2B Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein Apoptotic cysteine protease Apoptotic protease Mch-5 Apoptotic protease Mch5 CAP4 CASP-8 CASP8 CASP8_HUMAN Caspase 8 Caspase 8 apoptosis related cysteine peptidase Caspase-8 subunit p10 CED 3 FADD Like ICE FADD-homologous ICE/CED-3-like protease FADD-like ICE FLICE FLJ17672 ICE-like apoptotic protease 5 MACH alpha 1/2/3 protein MACH MACH beta 1/2/3/4 protein MCH5 MGC78473 MORT1 associated ced 3 homolog MORT1-associated CED-3 homolog OTTHUMP00000163717 OTTHUMP00000163720 OTTHUMP00000163724 OTTHUMP00000163725 OTTHUMP00000165062 OTTHUMP00000165063 OTTHUMP00000165064 OTTHUMP00000206552 OTTHUMP00000206582
Images
ET1612-70_1.jpg Fig1: Western blot analysis of Caspase-8 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1612-70_2.jpg Fig2: ICC staining of Caspase-8 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-70_3.jpg Fig3: ICC staining of Caspase-8 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.