Phospho-AMPK alpha 1 (S496) Recombinant Rabbit Monoclonal Antibody [SD0810]
cat.: ET1612-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | SD0810 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1 aa 471-520 / 559. |
| Positive control: | SiHa cell lysate, HUVEC cell lysate, K-562 cell lysate, mouse hippocampus tissue, mouse cerebral cortex tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IP IHC-Fr |
1:500-1:1,000 Use at an assay dependent concentration. 1:100 |
| Uniprot #: | SwissProt: Q13131 Human | Q5EG47 Mouse |
| Alternative names: | 5 AMP activated protein kinase alpha 1catalytic subunit 5 AMP activated protein kinase catalytic alpha 1 chain 5' AMP activated protein kinase catalytic subunit alpha 1 5'-AMP-activated protein kinase catalytic subunit alpha-1 AAPK1 AAPK1_HUMAN ACACA kinase acetyl CoA carboxylase kinase AI194361 AI450832 AL024255 AMP -activate kinase alpha 1 subunit AMP-activated protein kinase, catalytic, alpha -1 AMPK 1 AMPK alpha 1 AMPK alpha 1 chain AMPK AMPK subunit alpha-1 AMPK1 AMPKa1 AMPKalpha1 C130083N04Rik cb116 EC 2.7.11.1 HMG CoA reductase kinase HMGCR kinase hormone sensitive lipase kinase Hydroxymethylglutaryl CoA reductase kinase im:7154392 kinase AMPK alpha1 MGC33776 MGC57364 OTTHUMP00000161795 OTTHUMP00000161796 PRKAA 1 PRKAA1 Protein kinase AMP activated alpha 1 catalytic subunit SNF1-like protein AMPK SNF1A Tau protein kinase PRKAA1 wu:fa94c10 |
Images
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Fig1:
Western blot analysis of Phospho-AMPK alpha 1 (S496) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (ET1612-72) at 1/5,000 dilution. Lane 1: SiHa cell lysate Lane 2: HUVEC cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-72) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospho-AMPK alpha 1(S496) on HUVEC cell lysates. Lane 1: HUVEC cells, whole cell lysate, 10ug/lane Lane 2/3: HUVEC cells treated with UV(2 hours recovery), whole cell lysates, 10ug/lane Lane 4: HUVEC cells treated with UV(2 hours recovery), then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-AMPK alpha 1(S496) antibody (ET1612-72) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size:64 kDa Observed band size:64 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 14 seconds |
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Fig3:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (ET1612-72). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-72, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig4:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (ET1612-72). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-72, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.