Phospho-CDC37 (S13) Recombinant Rabbit Monoclonal Antibody [SD08-50]
cat.: ET1612-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: SD08-50
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37.
Positive control: HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, PC-12 cell lysate, HEK-293 cell lysate treated with λpp for 1 hour, C2C12 cell lysate treated with λpp for 1 hour.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IP

1:500-1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q16543 Human | Q61081 Mouse | Q63692 Rat
Alternative names: CDC 37 Cdc37 CDC37 cell division cycle 37 homolog CDC37 cell division cycle 37 S cerevisiae homolog CDC37 cell division cycle 37, S cerevisiae, homolog of Cdc37 homolog CDC37 protein CDC37_HUMAN CDC37A cell division cycle 37 Cell division cycle 37 homolog Hsp90 chaperone protein kinase targeting subunit Hsp90 chaperone protein kinase targeting subunit p50Cdc37 Hsp90 chaperone protein kinase-targeting subunit Hsp90 co chaperone Cdc37 Hsp90 co-chaperone Cdc37 p50 p50Cdc37 S cerevisiae hypothetical protein CDC37
Images
ET1612-74_1.jpg Fig1: Western blot analysis of Phospho-CDC37 (S13) on different lysates with Rabbit anti-Phospho-CDC37 (S13) antibody (ET1612-74) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: C2C12 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: HEK-293 cell lysate, treated with λpp for 1 hour
Lane 6: C2C12 cell lysate, treated with λpp for 1 hour

Lysates/proteins at 15 µg/Lane.

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-74) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.