| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SD08-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, PC-12 cell lysate, HEK-293 cell lysate treated with λpp for 1 hour, C2C12 cell lysate treated with λpp for 1 hour. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q16543 Human | Q61081 Mouse | Q63692 Rat |
| Alternative names: | CDC 37 Cdc37 CDC37 cell division cycle 37 homolog CDC37 cell division cycle 37 S cerevisiae homolog CDC37 cell division cycle 37, S cerevisiae, homolog of Cdc37 homolog CDC37 protein CDC37_HUMAN CDC37A cell division cycle 37 Cell division cycle 37 homolog Hsp90 chaperone protein kinase targeting subunit Hsp90 chaperone protein kinase targeting subunit p50Cdc37 Hsp90 chaperone protein kinase-targeting subunit Hsp90 co chaperone Cdc37 Hsp90 co-chaperone Cdc37 p50 p50Cdc37 S cerevisiae hypothetical protein CDC37 |
|
Fig1:
Western blot analysis of Phospho-CDC37 (S13) on different lysates with Rabbit anti-Phospho-CDC37 (S13) antibody (ET1612-74) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: PC-12 cell lysate Lane 5: HEK-293 cell lysate, treated with λpp for 1 hour Lane 6: C2C12 cell lysate, treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-74) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |