Phospho-Raf1 (S43) Recombinant Rabbit Monoclonal Antibody [SD087-05]
cat.: ET1612-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD087-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser43 of Human Raf1 aa 20-69 / 648. |
| Positive control: | NIH/3T3 cell lysate, 293T cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P04049 Human | Q99N57 Mouse | P11345 Rat |
| Alternative names: | c Raf C-raf C-Raf proto-oncogene, serine/threonine kinase CMD1NN Craf 1 transforming gene cRaf Craf1 transforming gene EC 2.7.11.1 kinase Raf1 Murine sarcoma 3611 oncogene 1 NS5 Oncogene MIL Oncogene RAF1 OTTHUMP00000160218 OTTHUMP00000207813 OTTHUMP00000209389 Protein kinase raf 1 Proto-oncogene c-RAF Raf 1 Raf 1 proto oncogene serine/threonine kinase RAF Raf proto oncogene serine/threonine protein kinase RAF proto-oncogene serine/threonine-protein kinase RAF-1 RAF1 RAF1_HUMAN Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV v raf 1 murine leukemia viral oncogene homolog 1 v-raf murine sarcoma viral oncogene homolog 1 v-raf-1 murine leukemia viral oncogene-like protein 1 vraf1 murine leukemia viral oncogene homolog 1 |
Images
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Fig1:
Western blot analysis of Phospho-Raf1 (S43) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: 293T cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.