Anti-SOX11 antibody [SD203-08]
cat.: ET1612-76
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IP
Clonality: Monoclonal
Clone number: SD203-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SOX11 aa 1-50 / 441.
Positive control: Human brain tissue lysates, rat spinal cord tissue lysates, SH-SY5Y, N2A.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: P35716 Human | Q7M6Y2 Mouse | P0C1G9 Rat
Alternative names: MRD27 SOX11 SOX11_HUMAN SRY (sex determining region Y) box 11 SRY related HMG box gene 11 SRY-box 11 Transcription factor SOX-11
Images
ET1612-76_1.jpg Fig1: Western blot analysis of SOX11 on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1612-76_2.jpg Fig2: Western blot analysis of SOX11 on rat spinal cord tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1612-76_3.jpg Fig3: ICC staining of SOX11 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-76_4.jpg Fig4: ICC staining of SOX11 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.