Cytokeratin 9 Recombinant Rabbit Monoclonal Antibody [SD201-09]
cat.: ET1612-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SD201-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein wiehin Human Cytokeratin 9 aa 234-422 / 623. |
| Positive control: | A431 cell lysates, mouse skin tissue, human breast tissue, MCF-7. |
| Subcellular location: | Extracellular exosome, membrane, nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IF-Cell |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50 |
| Uniprot #: | SwissProt: P35527 Human | Q6RHW0 Mouse | Q8CIS9 Rat |
| Alternative names: | CK 9 CK9 CK-9 Cytokeratin 9 Cytokeratin-9 Cytokeratin9 EPPK K1C9_HUMAN K9 Keratin 9 Keratin Keratin type I cytoskeletal 9 Keratin-9 Keratin9 KRT 9 Krt9 Spermatid Perinuclear Ring Manchette Protein K9 type I cytoskeletal 9 |
Images
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Fig1: Western blot analysis of Cytokeratin 9 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 9 (ET1612-77) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 9 (ET1612-77, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) and Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 9 (ET1612-77). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 9 (ET1612-77, red) at 1/50 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 9 (ET1612-77). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody Cytokeratin 9 (ET1612-77, red) at 1/200 dilution for overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/800 dilution. DAPI was used to stain the cell nuclei (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.