SOX10 Recombinant Rabbit Monoclonal Antibody [SD204-04]
cat.: ET1612-79
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: SD204-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide Human SOX10 aa 411-460 / 466.
Positive control: SK-MEL-28 cell lysate, B16-F1 cell lysate, A375 cell lysate, rat brain tissue lysate, rat brain tissue, human breast tissue, human skin tissue, mouse brain tissue, rat hippocampus tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:5,000
1:50-1:800
1:50
Uniprot #: SwissProt: P56693 Human | Q04888 Mouse | O55170 Rat
Alternative names: DOM DOM Dominant megacolon mouse human homolog of MGC15649 PCWH SOX 10 SOX10 SOX10_HUMAN SRY (sex determining region Y) box 10 SRY (sex determining region Y) box 10 SRY box 10 SRY box containing gene 10 SRY related HMG box gene 10 SRY related HMG box gene 10 Transcription factor SOX 10 Transcription factor SOX-10 WS2E WS4 WS4C
Images
ET1612-79_1.jpg Fig1: Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (ET1612-79) at 1/5,000 dilution.

Lane 1: SK-MEL-28 cell lysate
Lane 2: B16-F1 cell lysate
Lane 3: A375 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 60/75 kDa

Exposure time: 1 minute 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-79) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-79_2.jpg Fig2: Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (ET1612-79) at 1/5,000 dilution.

Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 50 kDa
Observed band size: 60/75 kDa

Exposure time: 25 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-79) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-79_3.jpg Fig3: Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (ET1612-79) at 1/5,000 dilution.

Lane 1: SK-MEL-28-si NT cell lysate
Lane 2: SK-MEL-28-si SOX10 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 60/75 kDa

Exposure time: 21 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-79) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-79_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-79_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOX10 antibody (ET1612-79) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-79) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-79_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-SOX10 antibody (ET1612-79) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-79) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-79_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-79_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-SOX10 antibody (ET1612-79) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-79) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-79_9.jpg Fig9: Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling SOX10 with Rabbit anti-SOX10 antibody (ET1612-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-79, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.