Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053]
cat.: ET1612-8
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SD2053 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 52/49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cyclin A1 aa 253-440 / 465. |
| Positive control: | Hela cell lysate, Huh7 cell lysate, Jurkat cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P20248 Human | P78396 Human |
| Alternative names: | CCN1 CCNA CCNA1 CCNA2 CT146 Cyclin-A Cyclin-A1 Cyclin-A2 |
Images
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Fig1:
Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates with Rabbit anti-Cyclin A1+ Cyclin A2 antibody (ET1612-8) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Huh7 (Human liver cancer cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1612-8, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48.6 kDa Observed band size: 55 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cyclin A1+ Cyclin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.