RON Recombinant Rabbit Monoclonal Antibody [SD2006]
cat.: ET1612-80
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: SD2006
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 152 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RON aa 11-60 / 1,400.
Positive control: AGS cell lysates, A549, AGS, A431, human lung carcinoma tissue, human breast carcinoma tissue, THP-1.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q04912 Human
Alternative names: c met related tyrosine kinase CD136 CD136 antigen CDw136 Macrophage stimulating 1 receptor (c met related tyrosine kinase) Macrophage stimulating 1 receptor Macrophage stimulating protein receptor alpha chain MACROPHAGE STIMULATING PROTEIN RECEPTOR Macrophage stimulating protein receptor beta chain Macrophage-Stimulating 1 Receptor (MST1R) Macrophage-stimulating protein receptor beta chain MSP receptor Mst1r MST1R variant RON30 MST1R variant RON62 NPCA3 p185 RON p185-Ron Protein-tyrosine kinase 8 PTK 8 ptk8 PTK8 protein tyrosine kinase 8 Recepteur d’origine nantais (RON) RON RON protein tyrosine kinase RON variant E2E3 RON_HUMAN Soluble RON variant 1 Soluble RON variant 2 Soluble RON variant 3 Soluble RON variant 4 Stem cell derived tyrosine kinase
Images
ET1612-80_1.jpg Fig1: Western blot analysis of RON on AGS cell lysates with Rabbit anti-RON antibody (ET1612-80) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 152 kDa
Observed band size: 152 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1612-80_2.jpg Fig2: ICC staining of RON in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-80_3.jpg Fig3: ICC staining of RON in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-80_4.jpg Fig4: ICC staining of RON in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-80_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-RON antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-80_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RON antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-80_7.jpg Fig7: Flow cytometric analysis of RON was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-80, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.