ET1612-82

Ubiquitin-like modifier-activating enzyme 1 Recombinant Rabbit Monoclonal Antibody [SD08-62]

cat.: ET1612-82

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality:	Monoclonal
Clone number:	SD08-62

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 118 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human UBA1 aa 1,009-1,058 / 1,058.
Positive control:	Mouse kidney tissue lysate, mouse liver tissue lysate, Hela, SKOV-3, A549, mouse ovary tissue, human kidney tissue, K562.
Subcellular location:	Nucleus, Mitochondrion, Cytoplasm.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:200 1:100-1:500 1:50-1:100
Uniprot #:	SwissProt: P22314 Human \| Q02053 Mouse \| Q5U300 Rat
Alternative names:	A1S9 A1S9 protein A1S9T and BN75 temperature sensitivity complementing A1S9T A1ST AMCX1 CFAP124 CTD-2522E6.1 GXP 1 GXP1 MGC4781 POC20 POC20 centriolar protein homolog Protein A1S9 SMAX2 Uba1 UBA1, ubiquitin-activating enzyme E1 homolog A UBA1_HUMAN UBA1A UBE 1 UBE 1X UBE1 UBE1X Ubiquitin activating enzyme E1 Ubiquitin-activating enzyme E1 Ubiquitin-like modifier-activating enzyme 1

Images

	Fig1: Western blot analysis of Ubiquitin-like modifier-activating enzyme 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse kidney tissue lysate Lane 2: Mouse liver tissue lysate
	Fig2: ICC staining of Ubiquitin-like modifier-activating enzyme 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of Ubiquitin-like modifier-activating enzyme 1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: ICC staining of Ubiquitin-like modifier-activating enzyme 1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Ubiquitin-like modifier-activating enzyme 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1612-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin-like modifier-activating enzyme 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1612-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of Ubiquitin-like modifier-activating enzyme 1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-82, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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