CDC23 Recombinant Rabbit Monoclonal Antibody [SD08-60]
cat.: ET1612-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD08-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cdc23 aa 521-570 / 597. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9UJX2 Human | Q8BGZ4 Mouse Unigene: 139314 Rat |
| Alternative names: | ANAPC8 Anaphase promoting complex subunit 8 Anaphase-promoting complex subunit 8 Apc 8 APC8 Cdc 23 CDC23 CDC23_HUMAN cell division cycle 23 Cell division cycle 23 homolog Cell division cycle protein 23 Cell division cycle protein 23 homolog Cut23 Cyclosome subunit 8 |
Images
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Fig1:
Western blot analysis of CDC23 on different lysates with Rabbit anti-CDC23 antibody (ET1612-84) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 69 kDa Observed band size: 56 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.