CDC40 Recombinant Rabbit Monoclonal Antibody [SD085-9]
cat.: ET1612-85
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SD085-9
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CDC40 aa 41-90 / 579.
Positive control: K-562 cell lysate, HCT 116 cell lysate, Neuro-2a cell lysate, Mouse lung tissue lysate, Rat brain tissue lysate, Neuro-2a, C6, rat brain tissue, mouse colon tissue, mouse brain tissue.
Subcellular location: Nucleus, Nucleus speckle.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:5,000
1:100
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O60508 Human | Q9DC48 Mouse
Entrez Gene: 361859 Rat
Alternative names: Cdc40 Cell division cycle 40 Cell division cycle 40 homolog EH binding protein 3 EH-binding protein 3 Ehb3 hPRP17 Pre mRNA processing factor 17 Pre mRNA splicing factor 17 Pre-mRNA-processing factor 17 PRP17 PRP17 homolog PRP17_HUMAN PRPF17
Images
ET1612-85_1.jpg Fig1: Western blot analysis of CDC40 on different lysates with Rabbit anti-CDC40 antibody (ET1612-85) at 1/5,000 dilution.

Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HCT 116 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 18 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-85) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-85_2.jpg Fig2: Immunocytochemistry analysis of Neuro-2a cells labeling CDC40 with Rabbit anti-CDC40 antibody (ET1612-85) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC40 antibody (ET1612-85) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1612-85_3.jpg Fig3: Immunocytochemistry analysis of C6 cells labeling CDC40 with Rabbit anti-CDC40 antibody (ET1612-85) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC40 antibody (ET1612-85) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1612-85_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
ET1612-85_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
ET1612-85_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.