APC6 Recombinant Rabbit Monoclonal Antibody [SD085-8]
cat.: ET1612-86
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD085-8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human APC6 aa 181-247 / 620. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, Hela cells lysates. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q13042 Human | Q8R349 Mouse Unigene: 66098 Rat |
| Alternative names: | ANAPC6 Anaphase promoting complex subunit 6 Anaphase-promoting complex subunit 6 Apc 6 APC6 CDC 16 CDC16 (cell division cycle 16 S. cerevisiae homolog) Cdc16 CDC16 homolog CDC16 protein CDC16_HUMAN CDC16Hs Cell division cycle 16 Cell division cycle 16 homolog Cell division cycle protein 16 homolog CUT9 Cyclosome subunit 6 |
Images
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Fig1:
Western blot analysis of APC6 on different lysates with Rabbit anti-APC6 antibody (ET1612-86) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 72 kDa Observed band size: 66 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.