Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat, Mouse |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | SD85-07 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 73 kDa |
Isotype: | IgG |
Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser259 of Human Raf1 aa 236-285 / 648. |
Positive control: | Hela cell lysates, rat skeletal muscle tissue, human testis tissue, human tracheal tissue. |
Subcellular location: | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
Uniprot #: | SwissProt: P04049 Human | Q99N57 Mouse | P11345 Rat |
Alternative names: | c Raf C-raf C-Raf proto-oncogene, serine/threonine kinase CMD1NN Craf 1 transforming gene cRaf Craf1 transforming gene EC 2.7.11.1 kinase Raf1 Murine sarcoma 3611 oncogene 1 NS5 Oncogene MIL Oncogene RAF1 OTTHUMP00000160218 OTTHUMP00000207813 OTTHUMP00000209389 Protein kinase raf 1 Proto-oncogene c-RAF Raf 1 Raf 1 proto oncogene serine/threonine kinase RAF Raf proto oncogene serine/threonine protein kinase RAF proto-oncogene serine/threonine-protein kinase RAF-1 RAF1 RAF1_HUMAN Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV v raf 1 murine leukemia viral oncogene homolog 1 v-raf murine sarcoma viral oncogene homolog 1 v-raf-1 murine leukemia viral oncogene-like protein 1 vraf1 murine leukemia viral oncogene homolog 1 |
Fig1:
Western blot analysis of Phospho-Raf1 (S259) on Hela cell lysates with Rabbit anti-Phospho-Raf1 (S259) antibody (ET1612-87) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 65 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-87) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Phospho-Raf1 (S259) antibody (ET1612-87) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-87) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-Raf1 (S259) antibody (ET1612-87) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-87) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig4:
Immunohistochemical analysis of paraffin-embedded human tracheal tissue with Rabbit anti-Phospho-Raf1 (S259) antibody (ET1612-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |