14-3-3 gamma Recombinant Rabbit Monoclonal Antibody [SD20-65]
cat.: ET1612-9
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD20-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 14-3-3 gamma aa 131-180 / 247. |
| Positive control: | 293T cell lysate, K562 cell lysate, A431 cell lysate, Hela cell lysate, K562, human breast cancer tissue, human lung cancer tissue, human ovary cancer tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC IHC-P |
1:1,000-1:2,000 1:10-1:50 1:20-1:1,000 |
| Uniprot #: | SwissProt: P61981 Human | P61982 Mouse | P61983 Rat |
| Alternative names: | 14 3 3 gamma 14 3 3 protein gamma 14 3 3 protein gamma subtype 14 3 3gamma 14-3-3 protein gamma 1433G_HUMAN 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide KCIP 1 KCIP-1 KCIP1 N-terminally processed Protein kinase C inhibitor protein 1 Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide Ywhag |
Images
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Fig1:
Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: Flow cytometric analysis of 14-3-3 gamma was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.