HDAC8 Recombinant Rabbit Monoclonal Antibody [SD082-07]
cat.: ET1612-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | SD082-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HDAC8 aa 61-110 / 377. |
| Positive control: | K562 cell lysate, Hela cell lysate, K562. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB FC IP |
1:500-1:2,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9BY41 Human | Q8VH37 Mouse | B1WC68 Rat |
| Alternative names: | CDA07 CDLS5 HD 8 HD8 HDAC 8 Hdac8 HDAC8_HUMAN HDACL 1 HDACL1 Histone deacetylase 8 Histone deacetylase like 1 MRXS6 RPD 3 RPD3 WTS |
Images
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Fig1:
Western blot analysis of HDAC8 on different lysates with Rabbit anti-HDAC8 antibody (ET1612-90) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of HDAC8 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.