Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
cat.: ET1612-91
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SD83-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 109 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Collagen VI alpha1 aa 17-255 / 1,028.
Positive control: NIH/3T3 cell lysate, human kidney tissue lysate, rat heart tissue lysate, rat kidney tissue lysate, Hela, A549, HepG2, RH-35, human lung tissue, human liver tissue, mouse colon tissue, human colon tissue, human skin tissue.
Subcellular location: Extracellular matrix.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:2,000
1:100-1:500
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: P12109 Human | P12110 Human | P12111 Human | Q02788 Mouse | Q04857 Mouse
Unigene: 232118 Rat
Alternative names: Alpha 1 (VI) chain (61 AA) CO6A1_HUMAN COL6A1 Collagen alpha-1(VI) chain Collagen type VI alpha 1 Collagen VI alpha 1 polypeptide CollagenVI Human mRNA for collagen VI alpha 2 C terminal globular domain OPLL PP3610
Images
ET1612-91_1.jpg Fig1: Western blot analysis of Collagen VI on different lysates with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate (15 µg/Lane)
Lane 2: Human kidney tissue lysate (20 µg/Lane)
Lane 3: Rat heart tissue lysate (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 109 kDa
Observed band size: 145 kDa

Exposure time: 1 minute 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-91) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1612-91_2.jpg Fig2: ICC staining of Collagen VI in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-91_3.jpg Fig3: ICC staining of Collagen VI in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-91_4.jpg Fig4: ICC staining of Collagen VI in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-91_5.jpg Fig5: ICC staining of Collagen VI in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-91_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-91_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-91_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-91_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-91_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1612-91_11.jpg Fig11: Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-91, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1612-91_12.jpg Fig12: Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody (ET1612-91) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-91, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
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