Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]
cat.: ET1612-94
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SD85-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cdc27 aa 662-824 / 824. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, PC-12 cell lysate, Rat thymus tissue lysate, Neuro-2a cell lysate, F9 cell lysate, human colon carcinoma tissue, human prostate tissue, human breast carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, spindle. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: P30260 Human | A2A6Q5 Mouse | Q4V8A2 Rat |
| Alternative names: | ANAPC3 Anaphase Promoting Complex 3 Anaphase promoting complex protein 3 Anaphase Promoting Complex Subunit 3 Anaphase-promoting complex subunit 3 APC 3 APC3 Cdc 27 Cdc27 CDC27 homolog CDC27_HUMAN CDC27Hs Cell Division Cycle 27 Cell division cycle protein 27 homolog D0S1430E D17S978E H NUC H-NUC HNUC Nuc2 homolog |
Images
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Fig1:
Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-94) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/5,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: F9 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-94) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.