CDC42EP1 Recombinant Rabbit Monoclonal Antibody [SD08-54]
cat.: ET1612-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SD08-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CDC42EP1 aa 310-359 / 391. |
| Positive control: | HeLa cell lysate, HUVEC cell lysate, HEK-293 cell lysate, K-562 cell lysate. |
| Subcellular location: | Endomembrane system, Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q00587 Human |
| Alternative names: | 55 kDa bone marrow stromal/endothelial cell protein Binder of Rho GTPases 5 Bone marrow stromal/endothelial cell protein, 55-KD BORG 5 BORG5 BORG5_HUMAN CDC42 effector protein (Rho GTPase binding) 1 Cdc42 effector protein 1 Cdc42ep1 CEP 1 CEP1 MGC15316 MSE 55 MSE55 OTTHUMP00000028709 Serum constituent protein Serum protein MSE55 |
Images
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Fig1:
Western blot analysis of CDC42EP1 on different lysates with Rabbit anti-CDC42EP1 antibody (ET1612-95) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HUVEC cell lysate Lane 3: HEK-293 cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 60 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.