14-3-3 Theta Recombinant Rabbit Monoclonal Antibody [SD084-02]
cat.: ET1612-97
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: SD084-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human 14-3-3 Theta aa 1-50 / 245.
Positive control: MCF-7 cell lysate, Hela cell lysate, Hela, A431, A549, SH-SY5Y.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  FC

1:500-1:5,000
1:100-1:500
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P27348 Human | P68254 Mouse | P68255 Rat
Alternative names: 14-3-3 14-3-3 protein T-cell 14-3-3 protein tau 14-3-3 protein theta 1C5 HS1 theta polypeptide tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Images
ET1612-97_1.jpg Fig1: Western blot analysis of 14-3-3 Theta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
ET1612-97_2.jpg Fig2: ICC staining of 14-3-3 Theta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-97_3.jpg Fig3: ICC staining of 14-3-3 Theta in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-97_4.jpg Fig4: ICC staining of 14-3-3 Theta in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1612-97_5.jpg Fig5: Flow cytometric analysis of 14-3-3 Theta was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-97, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.