Cdc34 Recombinant Rabbit Monoclonal Antibody [JJ086-07]
cat.: ET1701-10
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ086-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cdc34 aa 1-236 / 236. |
| Positive control: | 293 cell lysate, K562 cell lysate, Hela, A549, N2A, Jurkat. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC IF-Cell IF-Tissue IP |
1:1,000-1:5,000 1:50-1:100 1:100-1:500 1:100-1:500 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P49427 Human | Q8CFI2 Mouse Unigene: 2427 Rat |
| Alternative names: | Cdc 34 Cdc34 Cell division cycle 34 Cell division cycle 34 homolog (S. cerevisiae) Cell division cycle 34 homolog E2 CDC34 UB2R1_HUMAN UBC 3 UBC3 UBCH3 UBE2 R1 UBE2R1 Ubiquitin carrier protein Ubiquitin conjugating enzyme Cdc34 Ubiquitin conjugating enzyme E2 32 kDa complementing Ubiquitin protein ligase Ubiquitin protein ligase R1 Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 Ubiquitin-protein ligase R1 |
Images
|
Fig1:
Western blot analysis of Cdc34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: K562 cell lysate |
|
Fig2: ICC staining of Cdc34 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of Cdc34 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: ICC staining of Cdc34 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig5: Flow cytometric analysis of Cdc34 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.