Hsp22 Recombinant Rabbit Monoclonal Antibody [JJ08-53]
cat.: ET1701-11
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JJ08-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Hsp22 aa 1-50 / 196.
Positive control: HepG2 cell lysate, mouse skeletal muscle tissue lysate, Hela, A431, SH-SY5Y, mouse brain tissue, rat skeletal muscle tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:100-1:500
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: Q9UJY1 Human | Q9JK92 Mouse | Q9EPX0 Rat
Alternative names: Alpha crystallin C chain Alpha-crystallin C chain Charcot Marie Tooth disease axonal type 2L Charcot Marie Tooth disease spinal CMT2L CRYAC DHMN 2 DHMN2 E2 induced gene 1 protein E2-induced gene 1 protein E2IG1 H11 Heat shock 22kDa protein 8 Heat shock 27kDa protein 8 Heat shock protein 22 Heat shock protein beta 8 Heat shock protein beta-8 Hereditary motor neuropathy distal HMN 2 HMN2 HMN2A HSB8 HSPB 8 HspB8 HSPB8_HUMAN OTTHUMP00000239768 Protein kinase H11 Small stress protein like protein HSP22 Small stress protein-like protein HSP22 Spinal muscular atrophy distal adult autosomal dominant
Images
ET1701-11_1.jpg Fig1: Western blot analysis of Hsp22 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Mouse skeletal muscle tissue lysate
ET1701-11_2.jpg Fig2: ICC staining of Hsp22 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-11_3.jpg Fig3: ICC staining of Hsp22 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-11_4.jpg Fig4: ICC staining of Hsp22 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-11_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-11_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Hsp22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.