Hsp22 Recombinant Rabbit Monoclonal Antibody [JJ08-53]
cat.: ET1701-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JJ08-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Hsp22 aa 1-50 / 196. |
| Positive control: | HepG2 cell lysate, mouse skeletal muscle tissue lysate, Hela, A431, SH-SY5Y. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q9UJY1 Human | Q9JK92 Mouse | Q9EPX0 Rat |
| Alternative names: | Alpha crystallin C chain Alpha-crystallin C chain Charcot Marie Tooth disease axonal type 2L Charcot Marie Tooth disease spinal CMT2L CRYAC DHMN 2 DHMN2 E2 induced gene 1 protein E2-induced gene 1 protein E2IG1 H11 Heat shock 22kDa protein 8 Heat shock 27kDa protein 8 Heat shock protein 22 Heat shock protein beta 8 Heat shock protein beta-8 Hereditary motor neuropathy distal HMN 2 HMN2 HMN2A HSB8 HSPB 8 HspB8 HSPB8_HUMAN OTTHUMP00000239768 Protein kinase H11 Small stress protein like protein HSP22 Small stress protein-like protein HSP22 Spinal muscular atrophy distal adult autosomal dominant |
Images
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Fig1:
Western blot analysis of Hsp22 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse skeletal muscle tissue lysate |
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Fig2: ICC staining of Hsp22 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Hsp22 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Hsp22 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.