Anti-Cytokeratin 2e antibody [JJ201-8]
cat.: ET1701-13
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JJ201-8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Cytokeratin 2e aa 270-450.
Positive control: Human skin tissue lysates, human tonsil tissue, human esophagus tissue.
Subcellular location: Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P35908 Human
Alternative names: CK 2e antibody Cytokeratin-2e antibody Epithelial keratin-2e antibody K2e antibody Keratin type II cytoskeletal 2 epidermal antibody Keratin-2 epidermis antibody KRT2 antibody KRT2A antibody KRT2E antibody Type-II keratin Kb2 antibody
Images
ET1701-13_1.jpg Fig1: Western blot analysis of Cytokeratin 2e on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1701-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 2e antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-13_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Cytokeratin 2e antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.