HSPA2 Recombinant Rabbit Monoclonal Antibody [JJ08-77]
cat.: ET1701-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ08-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HSPA2 aa 448-639 / 639. |
| Positive control: | MCF-7 cell lysate, Hela cell lysate, mouse testis tissue, rat testis tissue, human smooth muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:400-1:1,000 |
| Uniprot #: | SwissProt: P54652 Human | P17156 Mouse | P14659 Rat |
| Alternative names: | 70kDa Hcp70.2 Heat shock 70 kDa protein 2 Heat shock protein 2 Heat shock protein 70.2 Heat shock related 70 kDa protein 2 Heat shock-related 70 kDa protein 2 Heat-shock protein, 70-KD, 2 Heat-shock protein, 70-KD, 3 HSP70 2 HSP70 3 Hsp70-2 HSP70-3 HSP70.2 HSP70A2 HSP72 HSP72_HUMAN HSPA2 Hspt70 Hst70 MGC58299 MGC7795 MGC93458 OTTHUMP00000180664 Testis-specific heat shock protein-related |
Images
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Fig1:
Western blot analysis of HSPA2 on different lysates with Rabbit anti-HSPA2 antibody (ET1701-15) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-15, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 70.0 kDa Observed band size: 70 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-HSPA2 antibody (ET1701-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HSPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using anti-HSPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.