Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
Clonality: | Monoclonal |
Clone number: | JJ087-04 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 12 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Sumo 3 aa 11-60 / 103. |
Positive control: | SW480 cell lysates, Hela, A549, RH-35, mouse lung tissue, mouse skin tissue, human kidney tissue, mouse kidney tissue, rat skin tissue, mouse large intestine tissue. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P55854 Human | P61956 Human | P61957 Mouse | Q9Z172 Mouse | P61959 Rat | Q5XIF4 Rat |
Alternative names: | HSMT3 MGC117191 OTTHUMP00000115275 OTTHUMP00000115276 OTTHUMP00000115277 Sentrin 2 Small ubiquitin like modifier 2 Small ubiquitin related modifier 2 small ubiquitin-like modifier 3 small ubiquitin-related modifier 3 SMT3 homolog 1 SMT3 homolog 2 SMT3 suppressor of mif two 3 homolog 1 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) SMT3 suppressor of mif two 3 homolog 2 SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) SMT3 suppressor of mif two 3 homolog 3 SMT3A SMT3B SMT3H1 SMT3H2 Sumo2 Sumo3 Ubiquitin like protein SMT3A Ubiquitin like protein SMT3B |
Fig1: Western blot analysis of Sumo 2+3 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. | |
Fig2: ICC staining of Sumo 2+3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: ICC staining of Sumo 2+3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig4: ICC staining of Sumo 2+3 in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-17, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Sumo 2+3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Sumo 2+3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig7:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sumo 2+3 antibody (ET1701-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Sumo 2+3 antibody (ET1701-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig9:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Sumo 2+3 antibody (ET1701-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Sumo 2+3 antibody (ET1701-17) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |