A-RAF Recombinant Rabbit Monoclonal Antibody [JJ08-68]
cat.: ET1701-18
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ08-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human A-RAF aa 146-310 / 606. |
| Positive control: | Hela cell lysate, A431 cell lysate, human kidney tissue, human breast carcinoma tissue, HeLa. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:100 |
| Uniprot #: | SwissProt: P10398 Human | P04627 Mouse |
| Alternative names: | A raf 1 A Raf proto oncogene serine/threonine protein kinase ARAF 1 Araf ARaf proto oncogene serine/threonine protein kinase ARAF_HUMAN ARAF1 Oncogene Araf1 Oncogene PKS2 PKS 2 PKS PKS2 Proto oncogene Pks Proto-oncogene A-Raf Proto-oncogene A-Raf-1 Proto-oncogene Pks RAFA 1 RAFA1 Ras binding protein DA Raf Serine/threonine-protein kinase A-Raf v raf murine sarcoma 3611 viral oncogene homolog v raf murine sarcoma 3611 viral oncogene homolog 1 v raf oncogene homolog 1 (murine sarcoma 3611 virus) |
Images
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Fig1:
Western blot analysis of A-RAF on different lysates with Rabbit anti-A-RAF antibody (ET1701-18) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD A-RAF cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-18) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of A-RAF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Application: Flow Cytometry (Intra) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: ET1701-18(1/100, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.