Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, ICC/IF, IHC-P, IP |
Clonality: | Monoclonal |
Clone number: | JJ08-70 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Protein A purified. |
Molecular weight: | 27 kDa |
Isotype: | IgG |
Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser78 of human Hsp27. |
Positive control: | NIH/3T3, MCF-7, Hela, human breast carcinoma tissue, human heart tissue, human skeletal muscle tissue. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB ICC/IF IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 |
Uniprot #: | P04792(Human) |
Alternative names: | Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 27 antibody |
![]() |
Fig1:
Western blot analysis of Phospho-Hsp27(S78) on different lysates using anti-Phospho-Hsp27(S78) antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Human skeletal muscle |
![]() |
Fig2: ICC staining Phospho-Hsp27(S78) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
![]() |
Fig3: ICC staining Phospho-Hsp27(S78) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
![]() |
Fig4: ICC staining Phospho-Hsp27(S78) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
![]() |
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Hsp27(S78) antibody. Counter stained with hematoxylin. |