14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ083-6]
cat.: ET1701-2
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ083-6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human 14-3-3 aa 1-126 / 246. |
| Positive control: | Human skin tissue lysate, NIH/3T3 cell lysate, mouse skin tissue, SK-Br-3 cell lysates. |
| Subcellular location: | Cytoplasm, Melanosome, Vacuole membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P31946 Human | P63104 Human | P63101 Mouse | Q9CQV8 Mouse | P35213 Rat | P63102 Rat |
| Alternative names: | 14 3 3 14 3 3 protein beta 14 3 3 protein beta/alpha 14 3 3 protein zeta 14 3 3 zeta 14-3-3 protein beta/alpha 14-3-3 protein/cytosolic phospholipase A2 1433B_HUMAN GW128 HS1 KCIP 1 KCIP-1 MGC111427 MGC126532 MGC138156 N-terminally processed Protein 1054 Protein kinase C inhibitor protein 1 Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide Tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide YWHAB YWHAD YWHAZ |
Images
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Fig1:
Western blot analysis of 14-3-3 on different lysates with Rabbit anti-14-3-3 antibody (ET1701-2) at 1/5,000 dilution. Lane 1: Human skin tissue lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-2) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of 14-3-3 on SK-Br-3 cell lysates with Rabbit anti-14-3-3 antibody (ET1701-2) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-2) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.