|Product Type:||Recombinant Rabbit monoclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse, Rat|
Western blot analysis of 14-3-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Human tonsil tissue lysate
Lane 2: HT-29 cell lysate
Lane 3: NIH/3T3 cell lysate
|Fig2: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.|