Phospho-B Raf (T401) Recombinant Rabbit Monoclonal Antibody [JJ08-72]
cat.: ET1701-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ08-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr401 of Human B Raf aa 371-420 / 766. |
| Positive control: | Mouse testis tissue lysates, PC-12 cell lysates, PC-3M, MCF-7, PC-12. |
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IP |
1:500-1:2,000 1:100-1:500 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P15056 Human | P28028 Mouse Entrez Gene: 114486 Rat |
| Alternative names: | FLJ95109 94 kDa B raf protein B raf 1 B Raf proto oncogene serine threonine protein kinase B Raf proto oncogene, serine/threonine kinase B RAF1 B-Raf proto-oncogene serine/threonine-protein kinase (p94) BRAF 1 BRAF BRAF_HUMAN BRAF1 cRmil MGC126806 MGC138284 Murine sarcoma viral (v-raf) oncogene homolog B1 Murine sarcoma viral v raf oncogene homolog B1 NS7 Oncogen BRAF oncogene BRAF1 p94 Proto-oncogene B-Raf Proto-oncogene c-Rmil RAFB 1 RAFB1 RMIL Serine/threonine-protein kinase B-raf v raf murine sarcoma viral oncogene homolog B v raf murine sarcoma viral oncogene homolog B1 v-Raf murine sarcoma viral oncogene homolog B1 |
Images
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Fig1: Western blot analysis of Phospho-B Raf (T401) on mouse testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of Phospho-B Raf (T401) on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of Phospho-B Raf (T401) in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Phospho-B Raf (T401) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of Phospho-B Raf (T401) in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.