Raf1 Recombinant Rabbit Monoclonal Antibody [JJ086-06]
cat.: ET1701-21
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JJ086-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Raf1 aa 1-50/648.
Positive control: A375 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela, MCF-7.
Subcellular location: Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P04049 Human | Q99N57 Mouse | P11345 Rat
Alternative names: c Raf C-raf C-Raf proto-oncogene, serine/threonine kinase CMD1NN Craf 1 transforming gene cRaf Craf1 transforming gene EC 2.7.11.1 kinase Raf1 Murine sarcoma 3611 oncogene 1 NS5 Oncogene MIL Oncogene RAF1 OTTHUMP00000160218 OTTHUMP00000207813 OTTHUMP00000209389 Protein kinase raf 1 Proto-oncogene c-RAF Raf 1 Raf 1 proto oncogene serine/threonine kinase RAF Raf proto oncogene serine/threonine protein kinase RAF proto-oncogene serine/threonine-protein kinase RAF-1 RAF1 RAF1_HUMAN Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV v raf 1 murine leukemia viral oncogene homolog 1 v-raf murine sarcoma viral oncogene homolog 1 v-raf-1 murine leukemia viral oncogene-like protein 1 vraf1 murine leukemia viral oncogene homolog 1
Images
ET1701-21_1.jpg Fig1: Western blot analysis of Raf1 on different lysates with Rabbit anti-Raf1 antibody (ET1701-21) at 1/1,000 dilution.

Lane 1: A375 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1701-21_2.jpg Fig2: ICC staining of Raf1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-21_3.jpg Fig3: ICC staining of Raf1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.